Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent aspirin (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed the external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes, CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent cilostazol (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: Hepatology (Baltimore, Md.)

Article Title: Coagulopathy, Bleeding Events and Outcome According to Rotational Thromboelastometry in Patients with Acute Liver Injury/Failure

doi: 10.1002/hep.31767

Figure Lengend Snippet: Individual cells display the median and IQR values for the parameter on that day. Shading (light green to red) indicates the increasing proportion of subjects with an abnormal ROTEM parameter for that day compared to reference values. APAP, acetaminophen; non-APAP, non-acetaminophen, CT, clotting time; CFT, clot formation time; alpha, alpha angle; MCF, maximal clot firmness.

Article Snippet: ROTEM analyses were performed on the ROTEM delta ® device (TEM International GmbH, Munich, Germany; later, Instrumentation Laboratory, Bedford, MA), which was supplied to each study site specifically for this study.

Techniques: Coagulation

Journal: Hepatology (Baltimore, Md.)

Article Title: Coagulopathy, Bleeding Events and Outcome According to Rotational Thromboelastometry in Patients with Acute Liver Injury/Failure

doi: 10.1002/hep.31767

Figure Lengend Snippet: Individual cells display the median and IQR values for the parameter on that day. Shading (light green to red) indicates the proportion of subjects with an abnormal ROTEM parameter for that day compared to reference values. LT, liver transplantation; TFS, transplant-free survival, CT, clotting time; CFT, clot formation time; alpha, alpha angle; MCF, maximal clot firmness.

Article Snippet: ROTEM analyses were performed on the ROTEM delta ® device (TEM International GmbH, Munich, Germany; later, Instrumentation Laboratory, Bedford, MA), which was supplied to each study site specifically for this study.

Techniques: Transplantation Assay, Coagulation

Journal: Hepatology (Baltimore, Md.)

Article Title: Coagulopathy, Bleeding Events and Outcome According to Rotational Thromboelastometry in Patients with Acute Liver Injury/Failure

doi: 10.1002/hep.31767

Figure Lengend Snippet: Bleeding events were recorded daily for the first 7 days of admission and adjudicated by committee. Individual cells display the median and IQR values for the parameter on that day. Shading (light green to red) indicates the proportion of subjects with an abnormal ROTEM parameter for that day compared to reference values. CT, clotting time; CFT, clot formation time; alpha, alpha angle; MCF, maximal clot firmness.

Article Snippet: ROTEM analyses were performed on the ROTEM delta ® device (TEM International GmbH, Munich, Germany; later, Instrumentation Laboratory, Bedford, MA), which was supplied to each study site specifically for this study.

Techniques: Coagulation

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Abrogating fibrinolysis does not improve bleeding or rFVIIa/rFVIII treatment in a non-mucosal venous injury model in haemophilic rodents

doi: 10.1111/jth.14148

Figure Lengend Snippet: Schematic depiction and representative examples of rotational thromboelastometry (ROTEM) traces recorded during recalcification, tissue factor (TF) and TF/tissue-type plasminogen activator (t-PA) ROTEM assays with whole blood from treated FVIII−/− rats. (A) Schematic depiction of a ROTEM curve that demonstrates where clot initiation, propagation, stabilization, lysis and dissolution of a formed thrombus can be detected. (B) Representative examples of recorded ROTEM traces from the recalcified whole blood assay, which assesses global clot formation. (C) Representative examples of recorded ROTEM traces for the TF assay, which assesses global clot formation following activation of coagulation with TF. (D) Representative examples of ROTEM traces for the TF/t-PA assay, which assesses the formation and stability of clots following addition of t-PA to all blood samples. Each trace in (B), (C) and (D) shows the rat whole blood sample that best represented its treatment group on the basis of mean clotting time and maximum clot firmness results. rFVIIa, recombinant activated factor VII; TXA, tranexamic acid; WT, wild-type.

Article Snippet: Whole blood coagulation profiles were obtained by use of a ROTEM delta hemostasis analyzer (TEM International, Munich, Germany) and prewarmed plastic test cups.

Techniques: Lysis, Whole Blood Assay, Transcription Factor Assay, Activation Assay, Coagulation, Recombinant

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Early posttraumatic hemostasis is not changed after GPIIb/IIIa inhibition via tirofiban. The in vivo inhibition of GPIIb/IIIa via tirofiban prior to burn injury does not change parameters assessed by thromboelastometry. Clotting time, indicating the onset of clot formation, alpha-angle, indicating the velocity of clot formation, and maximum clot firmness (MCF), indicating the clot stability, were measured ( a – c ). Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Inhibition, In Vivo, Coagulation

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Disruption of fibrinogen annihilates effective hemostasis. The disruption of fibrinogen annihilates hemostasis but the fibrinogenic pathway seems to be less affected than platelets, as in the thromboelastometry analysis, the fib-tem® activation, which inhibits platelet contribution to the clot firmness, is slightly sustained compared to ex-tem® activation. Activation of coagulation was conducted with ex-tem® and fib-tem®. The graphs representing the clotting process in the control group ( a ) and in the fibrinogen depletion group ( b ) were displayed. The alpha-angle and maximum clot firmness were compared between all groups and interventions ( c – f ). Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Activation Assay, Coagulation

Journal: Immunologic Research

Article Title: Platelets differentially modulate CD4 + Treg activation via GPIIa/IIIb-, fibrinogen-, and PAR4-dependent pathways

doi: 10.1007/s12026-021-09258-5

Figure Lengend Snippet: Early posttraumatic hemostasis is not differed following PAR4 disruption. The hemostasis assessed by thromboelastometry early post burn injury is not affected by PAR4 disruption. The clotting time (CT) ( a ), alpha-angle ( b ), and the maximum clot firmness (MCF) ( c ) were depicted. Data are expressed as box plots of the median fluorescent intensity (MFI) ± Q1 and Q3, and whiskers indicate max and min data points. n = 6–8. * p < 0.05

Article Snippet: Following intracardial blood puncture, samples were analyzed via rotational thromboelastometry (ROTEM delta®) (Tem international GmbH, Munich, Germany).

Techniques: Coagulation